Western blots of LN taken from these mice showed that detection of the LC3 II isoform was increased in mice treated with metformin, an effect attenuated by verteporfin (Fig 6D), and these effects paralleled the augmented tumor protection, and CD8+ cytotoxicity in metformin treated mice (Fig 6A–6C), again effects abolished by verteporfin. Here, CD8A is linked to neoplasm.