Immunohistochemistry for GLB1 was performed, quantitated, and analyzed utilizing VectraTM, an automated imaging system, within the epithelial and the stromal compartments of the ADT-treated (54) and control final surgical specimens (94).[19] Histologically PCa after ADT displayed compressed, fused glands, and cells contain abundant clear vacuolated cytoplasm and shrunken nucleoli in some areas when compared to control tissues (Fig 1A, 1B, 1C and 1D). Here, GLB1 is linked to posterior cortical atrophy.