This study demonstrated that overexpression of miR‐96‐5p and miR‐149 downregulated GPC1 expression in HT‐29 and HCT‐116 cells and xenograft HT‐29 and HCT‐116 tumours as well as decreased the secretion of GPC1 positive exosomes and subsequently induced cell apoptosis and inhibited cell proliferation in vitro and tumour growth in vivo. Here, GPC1 is linked to neoplasm.