To determine the expression profiles of E2F family members in pancreatic cancer, RT-PCR analyses were performed and revealed that E2F1, E2F3, E2F4 and E2F5 were all expressed in HPNE/KrasG12V/shp16, AsPc-1 and Panc-28 cells, while E2F2 was detected only in AsPc-1 cells (Fig. 5b). This evidence concerns the gene E2F4 and familial pancreatic carcinoma.