Notably, of the top 5 differentially methylated regions (Table 5), ATP6V0E2 (R2 = 0.226) is an isoform of the H(+)-ATPase V0 e subunit, which is important for cellular energy [66], LOC401431 (R2 = 0.224) encodes the antisense RNA for ATP6V0E2 suggesting that the regulation dynamics of this particular gene may be affected in ME/CFS, IL6R (R2 = 0.220) encodes for the receptor of IL-6, a pleiotropic cytokine, and LOC144571 (R2 = 0.220) is the antisense RNA to alpha-2-macroglobulin, a protease inhibitor and cytokine transporter. This evidence concerns the gene A2M and myalgic encephalomeyelitis/chronic fatigue syndrome.