While GATA4 knockdown is embryolethal, mutations of GATA4 are associated with cardiac septal defects as well as pulmonary stenosis (Garg et al., 2003; Tomita-Mitchell et al., 2007; McCulley and Black, 2012; Misra et al., 2012), but also with tetralogy of Fallot (TOF) and double outlet right ventricle (DORV) (Andersen et al., 2014). Here, GATA4 is linked to heart septal defect.