As shown in the results for the 208 salivary samples collected from the sporadic moderate-to-profound hearing loss patients, GJB2 and SLC26A4 contributed most mutations, of which, 235delC in GJB2 (43/95, 45.3%) and c.919-2A > G in SLC26A4 (33/95, 34.7%) appeared to be the most common mutations. The gene discussed is GJB2; the disease is hearing loss disorder.