Results show that the frequency of NK1.1− CD4+ NKG2D+ T cells in colon is negatively correlated with colitis induced by DSS, and NK1.1− CD4+ NKG2D+ T cell differs from NK1.1+ CD4+ NKG2D+ T cells in terms of cell membrane markers and transcriptional RNAs. The gene discussed is CD4; the disease is colitis.