To determine whether influenza virus infection affects the status of the host SUMOylation, A549 cells were infected with a high pathogenic virus strain [A/environment/Qinghai/1/2008(H5N1)] at a multiplicity of infection (MOI) of 7, and virus-infected cells were collected at 4, 8, 12, and 24 h post infection (p.i.)and subjected to immunoblotting analysis against SUMO1, Ubc9, SAE1, SAE2, and GADPH specific monoclonal antibodies. Here, SAE1 is linked to infection.