To examine whether overexpression of MEK1 SUMOylation would alter the outcome of virus infection, we constructed a dicistronic plasmid, HA-MEK1-Ubc9, which contains a sequence coding for an N-terminally HA-tagged MEK1 protein, the internal ribosome entry sites (IRES) from the encephalomyocarditis virus (EMCV), and N-terminally Myc-tagged Ubc9 protein (Figures 2A,C). Here, MAP2K1 is linked to viral infectious disease.