Given HE3286's proposed anti-inflammatory action and the contribution of microglia as neuroinflammatory mediators in glaucoma, we examined how treatment influences levels of ionized calcium-binding adapter molecule 1 (Iba1), a microglia-specific marker (Ito et al., 1998; Soto and Howell, 2014; Mac Nair and Nickells, 2015). Here, AIF1 is linked to glaucoma.