Here, we report on differential T cell cytokine secretion at baseline and at week 6 (following systemic therapy with ipilimumab) in response to melanoma lineage (gp-100, MART-1) and cancer testis (NY-ESO 1) antigen stimulation in 13 patients treated with ipilimumab in the neoadjuvant setting. This evidence concerns the gene PMEL and melanoma.