Therefore, realizing that TLR2/9−/− mice were less effective in protecting against HSV-1 [8] and that they produced less IL-1β and iNOS, typical molecules in the Th1 immune response, we analyzed the mRNA levels of IL-10, a regulatory cytokine in the immune response, and IL-5, an important component of the Th2 immune response, on the 5th day post infection using qPCR. This evidence concerns the gene IL1B and infection.