ACTA1 and breast cancer: Staining was performed for the EMT marker α-SMA (Figures 6D-G, D’-G’), previously described in PECs in disease [2, 34-36], and was quantitated and expressed as the percentage of glomeruli with α-SMA staining by kidney region (OC and JM glomeruli), and within these glomeruli, by the intra-glomerular staining pattern along Bowman's capsule (BC), in the tuft only, and along both BC and in the tuft.