Similar to the OS analysis, we constructed a predictive model for AML transformation based on the significant gene mutations associated with AML transformation using the following definitions: Low: 0–1 common mutations (mutations in all genes except for DNMT3A, TP53, WT1, SRSF2, IDH1/2, STAG2 and ROBO1/2); Intermediate: 1 driving mutation (DNMT3A, TP53, WT1, SRSF2, IDH1/2, STAG2 and ROBO1/2) or 2 common mutations; High: ≥2 driving mutations or the presence of ≥3 common mutations (Supplemental Table 5). The gene discussed is DNMT3A; the disease is acute myeloid leukemia.