Regarding AML-free survival, univariate analyses showed that advanced age (P = 1.0E-03, HR = 1.026), a high IPSS-R score (P = 1.0E-13, HR = 1.709) and mutation of the DNMT3A (P = 3.1E-09, HR = 4.901), TP53 (P = 2.5E-05, HR = 3.875), WT1 (P = 2.0E-03, HR = 4.887), SRSF2 (P = 2.0E-03, HR = 3.687), IDH1/2 (P = 2.0E-03, HR = 2.891), STAG2 (P = 8.0E-03, HR = 3.139) and ROBO1/2 (P = 4.7E-02, HR = 1.833) genes predicted a high AML transformation rate (Fig. 4b). This evidence concerns the gene DNMT3A and acute myeloid leukemia.