In retinal pigment epithelium (RPE) cells, primary target cells for the treatment of AMD, AAV encoding the Vegfa-specific CjCas9 (AAV-CjCas9: Vegfa) achieved indels with frequencies that ranged from 22 to 30% at day 14, 28, and 42 post injection (Fig. 5c). This evidence concerns the gene VEGFA and age-related macular degeneration.