Considering the potential influence of ROCK inhibition on bladder overactivity and inflammatory cascade, here, we tested whether GSK 269962 would rescue cystometric and inflammatory changes in a model of cystitis induced by CYP injection, which is widely used for investigation of the pathophysiology, prevention, and treatment of HC as well as a model for bladder inflammation and OAB (Juszczak et al. 2010; Lee et al. 2014). The gene discussed is PPIG; the disease is cystitis.