In all NASH-associated biopsies and HCCs, we observed significant elevation of numerous extracellular matrix and cytoskeleton proteins, the majority of them being downstream of transforming growth factor β (TGF-β), including different types of collagens, fibronectin (FN), intermediate filament vimentin (VIM), β actin-like protein 2 (ACTB2), myosin 9 (MYH9), tropomyosin α-4 chain (TPMA4), tubulin α-1C chain (TUBA1C), moesin (MSN), lumican (LUM) and others (Table 1). The gene discussed is TGFB1; the disease is metabolic dysfunction-associated steatohepatitis.