In NASH HCCs, we further found significant elevation of cytochrome P450, family 51, subfamily A, polypeptide.1 (CYP51A1), cytochrome P450, family 4, subfamily F, polypeptide11 (CYP4F11) and cytochrome P450, family 8, subfamily B, polypeptide1 (CYP8B1), which are involved in lipid, cholesterol and bile acid biosynthesis, likely regulated through sterol regulatory element-binding proteins (SREBP)—liver X receptor α (LXRα) pathway. This evidence concerns the gene CYP51A1 and metabolic dysfunction-associated steatohepatitis.