Examination of altered canonical pathways by IPA demonstrated that many proteins with altered expression in NASH-associated biopsies and HCCs were related to fibrosis/hepatic stellate cell activation, lipid biogenesis, fatty acid β- and α-oxidation I, ethanol degradation II, and activation of Nrf2, SREBP, PI3K/AKT and nNOS signaling, gluconeogenesis and putrescine degradation III (Figure 1C and Table S5). This evidence concerns the gene NOS1 and metabolic dysfunction-associated steatohepatitis.