In this study, we investigated the effect of several key drugs used in the clinical treatment of lymphoma, including a cytotoxic agent [doxorubicin (ADM)], an antimetabolite drug (cytarabine, Ara‐C), DNA‐damaging drugs [CDDP, and oxaliplatin (Oxal)], a topoisomerase inhibitor [etopside (Etop)] and a alkaloid [vincristine (VCR)] on the expression of PD‐L1 in bone marrow stromal cells. The gene discussed is CD274; the disease is lymphoma.