To investigate whether PDL1 is expressed in tumor-associated LVs, we implanted VEGF-C overexpressing B16F10 melanoma cells [B16F10-VEGFC (22)] intradermally into syngeneic C57BL/6 mice, and analyzed the expression of PDL1 by immunofluorescence staining of tumor sections 2 weeks later. Here, CD274 is linked to melanoma.