Herein, we successfully generated Rp9 gene knockout (Rp9-KO) and point mutation knock in (Rp9-KI) (Rp9, c.A386T, P.H129L) which was analogous to the reported one in the retinitis pigmentosa patients (Rp9, c.A410T, P.H137L) in 661 W retinal photoreceptor cells by using CRISPR/Cas9-mediated approach. The gene discussed is RP9; the disease is retinitis pigmentosa.