Here, we applied Q-PCR to examine the cytokines/chemokines expression in the CD8+ T cell line – Molt-3 cells and the BPH epithelial cell line – Bph-1 cells, and the ELISA assay to identify the secretion of CCL5 in conditioned media at a low androgen level. The gene discussed is CCL5; the disease is benign prostatic hyperplasia.