To evaluate the potential role of Gal-1 and MDR1 in breast cancer, the expression levels of Gal-1 and MDR1 in MCF-10A, MCF-7, MCF-7/PTX, MCF-7/ADR cells, normal tissues and breast tumor tissues samples were detected by qRT-CPR and western blot. The gene discussed is LGALS1; the disease is breast neoplasm.