TP53 and liver cancer: In summary, we found that the liver carcinogen AFB1 induces >30 fold more meth-OH-PdG adducts, the major adducts formed by interactions of Acet and Cro and DNA, than AFB1-E-dG adducts in liver cells, that this type of adduct preferentially occurs at codon 249 of the p53 gene, the sole mutational hotspot in AFB1 related liver cancer, and that repair of DNA damage at codon 249 is slower and less efficient than at other codons.