RPS6KB1 and hydrops fetalis: Western blotting analysis showed the up-regulation of the phosphorylated forms of mTOR (p-mTOR) and ribosomal protein S6 kinase (p-p70S6K) in the Ath+HF+BCAA group, confirming the activation of mammalian target of rapamycin complex 1 (mTORC1) signaling by BCAA supplementation (Figure 2B).