TGFBR1 and hydrops fetalis: qRT-PCR analysis of TGFβR1 and TGFβR2 showed the significant up-regulation of TGFβR1 in the Ath+HF group, and its expression was significantly repressed in the Ath+HF+BCAA group at both 38w and 68w (Figure 4B), while no significant reduction of TGFβR2 was observed in the Ath+HF+BCAA group.