In this study with melanoma models, our data clearly show that down-regulated Gli-1 and Gli-2, and up-regulated Gli-3 in melanoma cells and mice treated with itraconazole are consistent with the reduced proliferation of melanoma cells and the smaller size of tumors in mice model, suggesting that itraconazole can serve as a potent inhibitor of Hh signaling to aid in melanoma therapy when combined with other drugs. This evidence concerns the gene GLI2 and melanoma.