We find that itraconazole can inhibit the activation of mTORC1 effectively in melanoma cells, indicated by decreased p-S6K and p-4E-BP1 levels in Western blots, and attenuate mTORC2 activity as well, indicated by less p-AKT-Ser473 under itraconazole treatment. This evidence concerns the gene EIF4EBP1 and melanoma.