Although itraconazole has a strong safety record for treatment of human diseases, to answer whether itraconazole given to xenograft melanoma mice in this experiment can cause cytotoxicity, the plasma from itraconazole free- and itraconazole treated-mice were subjected to a panel of organ functional tests including hepatic alanine aminotransferase [ALT], total bilirubin [TBIL], and alkaline phosphatase [ALKP] and kidney functional tests such as contents of creatinine, urea nitrogen [BUN], albumin, cholesterol, glucose, and calcium in the blood samples. This evidence concerns the gene GPT and melanoma.