In a subcutaneous xenograft model injected with TKKK cells, both the anti-tumor and anti-metastatic effect of vandetanib was demonstrated confirming the in vitro data [64] and suggesting that a particular setting of CCA, characterized by EGFR amplification and lack of K-RAS mutations, could benefit by the clinical employ of vandetanib. Here, EGFR is linked to cholangiocarcinoma.