EGF-treated 3D Caco-2 luminal cyst cultures had a statistically significant increase in glucose consumption compared to untreated models (6.492 ± 2.189 versus 1 ± 0.01663, n = 4, p = 0.0024, Fig. 2g), a statistically significant increase in lactate production (6952 ± 957 pmol versus 2578 ± 206 pmol, n = 4, p = 0.0001, Fig. 2h), and a statistically significant increase in relative cell ATP content (2.145 ± 0.246 versus 1 ± 0.41, n = 4, p = 0.0031, Fig. 2i). Here, EGF is linked to cyst.