The impact of hepatic fibrosis and steatosis on hepatic PAI-1 expression in CHC patients before anti-HCV therapy were negligible, as only some biliary cells and endothelial cells (Fig. 3A–D, black arrows), but no inflammatory cells or hepatocytes, expressed PAI-1, and the expression was very weak. The gene discussed is SERPINE1; the disease is Hepatic fibrosis.