To investigate the expression patterns of ZKSCAN1, HCC tissues and adjacent nontumorous tissues were subjected to reverse transcription PCR (RT‐PCR) and western blot analysis, and the results are shown in Fig. 1A. Both the mRNA and protein of ZKSCAN1 were significantly down‐regulated, indicating that the ZKSCAN1 gene may be involved in the progression of HCC. This evidence concerns the gene ZKSCAN1 and hepatocellular carcinoma.