To explore the underlying mechanism by which ZKSCAN1 regulated cell proliferation and invasion in HCC cells, we performed RNA‐seq analysis to identify changes in gene expression after knockdown of ZKSCAN1 mRNA (shZKSCAN1) or circZKSCAN1 (shcircZKSCAN1) in SMMC‐7721 cells. This evidence concerns the gene ZKSCAN1 and hepatocellular carcinoma.