CASP3 and neoplasm: Notably, the combination of TRAIL and TSN exhibited low toxicity in tumor-bearing mice, because the cleaved band of caspase 3 was not observed in the liver or spleen tissue of the TSN group or the TRAIL + TSN group (Fig. 8E), as demonstrated by the results of H&E staining and CL-caspase 3 immunohistochemistry analysis.