To study the impact of activation-associated Akt1 mutants on cell behaviour, we used retroviral gene transfer to generate murine Tramp-C1 prostate cancer cells (TrC1) and Akt1 knock-out (Akt1−/−) mouse embryonic fibroblasts (MEF) stably expressing specific Akt1-eGFP fusion proteins. Here, AKT1 is linked to prostate cancer.