Next, we detected the expression of FEZF1-AS1 in gastric cancer cells with si-SP1, pcDNA-SP1 and control, the results shown that relative expression of FEZF1-AS1was downregulated in AGS and SGC-7901cells with si-SP1 (Fig. 7a); however, expression of FEZF1-AS1was upregulated in AGS and 293 T cells with pcDNA-SP1 compared with pcDNA vector (Fig. 7b). Here, SP1 is linked to gastric cancer.