Also, KEGG pathway analysis revealed no results related to immune response consistently significant across all hepatosteatotic groups (S4 Table) and specific analysis of mRNA levels of markers for leukocytes (Cd45), T cells (Cd28, Csf2, Ccr5, Cxcr4) and B cells (Pax5, Cd79b) supported the absence of significant immune cell infiltration and NASH at this stage of NAFLD development. This evidence concerns the gene CXCR4 and metabolic dysfunction-associated steatohepatitis.