Flow cytometry analysis performed after 48 h of co-incubation revealed that nearly 70% of CD3+ T-cells expressed the specific activation markers CD25+ and CD69+ on their cell surface upon cross-linkage with tumor cells via MSC-released bsAb in both 3D samples (Fig. 7b) and in 2D samples performed in parallel as a control (Supplementary Fig. 5a). The gene discussed is CD69; the disease is neoplasm.