Furthermore, it has recently been reported that the Wnt family member, Wnt5A, promotes phosphorylation of Akt at Ser473 downstream of PI3K to promote osteosarcoma cell migration55, supporting the hypothesis that PAK4 regulation of Akt phosphorylation at Ser473 may also be required for PDAC cell migration. This evidence concerns the gene AKT1 and osteosarcoma.