Then, to evaluate if the release of such cytokines could contribute to the high cytotoxicity of CR-LAAO on tumor cells, we investigated the pro-apoptotic potential of this enzyme by staining with annexin V-FITC and PI conjugated to fluorescein, which is a marker of phosphatidylserine exposed by cells at different stages of apoptosis30. Here, ANXA5 is linked to neoplasm.