Although MVs shed by the human cervical carcinoma HeLa cell line did not have any detectable VEGF (Fig. 3e; Supplementary Fig. 3C); interestingly, we detected MVs with associated VEGF90K in other human cancer cell lines including U87 glioblastoma cells and HT29 colorectal adenocarcinoma cells (Supplementary Fig. 3F). This evidence concerns the gene VEGFA and colorectal adenocarcinoma.