More recently, the development of the RT-QuIC assay [5], an in vitro fluorimetric assay which is able to indirectly detect very low amounts of prions, based on the capacity of PrPSc to induce recombinant (rec-) PrP conversion and aggregation, has provided a very promising tool to improve the early diagnosis of human prion diseases in a noteworthy manner given its high degree of sensitivity (82–97%) and specificity (99–100%) [5, 19, 41, 45, 46, 55, 64]. Here, PRNP is linked to prion disease.