Additionally, HFD mice developed moderate liver fibrosis as measured by morphometric analysis of fibrotic areas in liver sections immune-stained with Sirius red and by assessment of the expression of αSMA and α1-Collagen, two pro-fibrogenetic genes (Fig. 1H and I; P < 0.01 versus naïve). The gene discussed is ACTA1; the disease is Hepatic fibrosis.