RT-PCR was used to quantify miR-17-5p levels in patient sera, showing that patients with a PD-L1+ tumor had lower levels of miR17-5p (mean±SD 34.1±3.4), compared to patients with a PD-L1- lesion (mean±SD 21.47±2.0, Figure 8D). The gene discussed is CD274; the disease is neoplasm.