Specifically, in 4 cases with PD-L1- pre-treatment biopsies (failing to reach the ≥5% threshold) upon resistance we observed a shift from PD-L1- to PD-L1+ status, while in the remaining cases PD-L1 was highly up-regulated in resistant melanoma tissues when compared with baseline, with diffuse and uniform membranous pattern of expression, irrespective from the presence of infiltrating immune cells (Figure 8B-8C and Supplementary Table 7). Here, CD274 is linked to melanoma.