The knockdown efficiency was 60–70%, measured by qRT-PCR, in 12 lung cancer cell lines (Fig. 3A and Supplementary Figure 1) with different genomic background (Supplementary Table 2) and different FAM83H-AS1 expression levels examined by RNA-Seq and RT-PCR (Supplementary Figure 2). The gene discussed is SACK1H; the disease is lung carcinoma.