NRAS and melanoma: Initially, we depleted Usp9x using a previously characterized shRNA knockdown (KD) vector40 in three melanoma cell lines with distinct driver mutations (BRAF mutant: SK-Mel28, A375; NRAS mutant: SK-Mel147) and metastatic efficiencies (highly metastatic: A375, SK-Mel147) and compared biological effects to control cells.