To determine whether this site affects Ets-1 tumorigenic activity, mutant Ets-1 (K388R) was expressed in melanoma with low endogenous Ets-1 expression (SK-Mel29; Fig. 4e), and tumorigenic activity was assessed by monitoring colony formation (Fig. 4f) or plating on matrigel (Fig. 4g). Here, ETS1 is linked to melanoma.