To further examine the therapeutic efficacy of systemic inhibition of Dub3 in preventing tumour recurrence and metastasis, we performed a spontaneous metastasis model analysis, in which control and DOX-inducible Dub3 shRNA MDA-MB231 cells were implanted into mammary fat pads of 6-week-old female SCID mice. This evidence concerns the gene USP17L2 and neoplasm.