DDX4 and cyst: Even though these excess cells were all positively stained with the germ cell marker Vasa in both Tj>E(Pc) dsRNA testes (Fig 2C, 2E and 2E”) and Tj>E(Pc) shRNA testes (S3A, S3B’,S3C and S3C” Fig), the early-stage cyst marker Zfh-1 was also detectable in these cells in 12.8% of Tj>E(Pc) dsRNA testes (N = 86, Fig 2E’–2E”) and 10% of Tj>E(Pc) shRNA testes (N = 79, S3C’–S3C” Fig).