LGALS4 and cyst: When E(Pc) was knocked down in cyst cells using a cyst cell-specific Tj-Gal4 driver [42] paired with two independent RNAi lines [E(Pc) dsRNA or E(Pc) shRNA, when phenotypes from these two lines are indistinguishable we called them E(Pc) RNAi, see Materials and Methods], the GFP signal representing the E(Pc) gDNA-GFP fusion protein level was greatly reduced in cyst cells compared to the neighboring germ cells (S1C–S1C” Fig), suggesting efficient knockdown.