DDX4 and cyst: Third, cells with both germline marker Vasa and early cyst cell marker Zfh-1 could be detected in 18% of Tj>Tip60 RNAi (N = 60, Fig 7G–7G”) and 19% of Tj>Tip60E431Q (N = 21, Fig 7I and 7I’) testes, respectively.