In summary, both cyst cell and germline defects in either Tj>Tip60 RNAi or Tj>Tip60E431Q testes were similar to those found in Tj>E(Pc) RNAi testes (Fig 1B–1E and Fig 2 and S3 Fig), suggesting that E(Pc) and Tip60 act together to regulate cyst cell differentiation cell-autonomously, as well as coordinate germ cell differentiation and maintain germline fate non-cell-autonomously. Here, KAT5 is linked to cyst.