In contrast, after overexpression of miR-34b-5p by miRNA mimic (Supplementary Figure 1A) and following inoculation with TCID50 of ALV-J, the expression level of MDA5 was downregulated (Supplementary Figure 1B) in miR-34b-5p overexpression DF-1 cells compared with negative control (NC) group at 2-48 h ALV-J infection time points. This evidence concerns the gene IFIH1 and infection.