The upregulation of another three known ESCC lncRNAs, comprising PCA1,24 TUG125 and H19,26 were also consistent with the upregulation reported by other groups.24, 25, 26 Interestingly, we found that two cancer-related lncRNAs PVT127 and WT1-AS,28 which were not reported in ESCC, displayed differential expression based on our RNA-seq analysis. Here, H19 is linked to cancer.