The induction of ICOSL expression, however, was significantly lower in most of the murine syngeneic tumour cell lines tested, including B16-F10 melanoma, MC38 and CT26 colon carcinoma, TRAMP C2 prostate carcinoma, ID8 ovarian carcinoma, 4T1 breast carcinoma, and MB49 bladder carcinoma (Fig. 2c). This evidence concerns the gene ICOSLG and urinary bladder carcinoma.