INS and type 2 diabetes mellitus: 2001). We observed in our study that HFD treatment along with intraperitoneal administration of STZ (40 mg/kg) damaged the insulin-secreting β-cells of the pancreas by breaking the DNA strand and caused depressed β-cell function, decreased endogenous insulin release and defects in insulin action (Koning et al. 2008). Other investigators have also used similar experimental T2DM animal model to explore drug discoveries (Zhang et al. 2010; Veerapur et al. 2012).