In the present study, we used several animal models including phenylephrine (PE)‐induced prostatic hyperplasia mice, a parabiosis mice model by Nestin‐Cre, Rosa26‐YFPflox/+mice surgically sutured with wild‐type littermates and conditional knockout Nestin‐Cre-ER, Tgfbr2flox/flox mice to investigate whether nestin+ MSCs are mobilized in peripheral circulation, recruited to the prostatic stroma and give rise to the fibroblasts during prostatic hyperplasia. This evidence concerns the gene NES and prostate disorder.