Thus, one would expect that if the effects of Q and ROE are no longer beneficial after 9 wks due to the lack of change in total Pgc-1α expression, the continuous impetus of the HFD would lead to similar skeletal muscle physiologies in HF, HF + Q, and HF + RO groups; yet at 9 wks, we still observed increased mitochondrial number [32] and decreased Pgc-1α methylation in HF + Q and HF + RO. Here, PPARGC1A is linked to hydrops fetalis.